It is unique among wild rice species, since it has. Plant promoter prediction tool and isolation of a promoter from porteresia coarctata mahalakshmi s1,krishna nc 2,rao kv 1,reddy vd 1 1 centre for plant molecular biology, osmania university, hyderabad, india and 2 sr software, jubilee hills, hyderabad, india abstract in silico identification of promoters is complicated by. While members of the genus oryza are very sensitive to salinity, salt concentrations as high as 20% of that of seawater had no adverse effect on the growth of the tropical wild rice porteresia coarctata tateoka in experiments undertaken in a greenhouse in the uk. Draft genome sequence of first monocothalophytic species oryza. Further details about the rice species and related genera are given in tables 11 and 12. Tateoka pcino1, af412340 earlier reported from this laboratory, has been analyzed by in vitro mutant and hybrid generation and subsequent biochemical and biophysical studies of the recombinant proteins. For full access to this pdf, sign in to an existing account, or purchase an annual subscription. Pdf in vitro propagation of salttolerant wild rice relative. Usda, ars, germplasm resources information network.
Porteresia coarctata triosephosphate isomerase pctpi mrna, complete cds. Regulatory region of ubiquitin 2 gene from porteresia coarctata makes efficient promoters for transgene expression in monocots and dicots. Full length cdna overexpression foxcox system was used to identify porteresia coarctata genes with positive involvement in salt stress response using arabidopsis model. The rice tribe, oryzeae, consists of 12 genera and is distributed in tropical and temperate regions worldwide clayton and renvoize, 1986.
Porteresia coarctata oryza coarctata is a wild halophyte, which shows. Merge pdf files combine pdfs in the order you want with the easiest pdf merger available. Estuarine macrophytes at bakkhali, coxs bazar, bangladesh. The habitat of porteresia coarctata from mandovi estuary, goa. Phylogeny of rice genomes with emphasis on origins. Functional screening of genes from a halophyte wild rice. Porteresia coarctata is a c4 grass which occupies a significant part of estuarine vegetation as mangrove associates in the eastern and western coasts of india. Northern analysis and in situ mrna hybridization re vealed the expression of pcsrp in the salt stressed roots and rhizome of p. Salt tolerant transgenic rice variety will be develop that ultimately benefits the farmers. Pdf merge combine pdf files free tool to merge pdf online. Effects of partial drying on seed germination in the aquatic grasses zizania palustris l. Variations in the protein content of porteresia coarctata may be due to difference in habitat and season14. Oryza coarctata also known as porteresia coarctata, lu and ge, 2003 is an allotetraploid wild rice with extreme salt and submergence tolerance. Problems and prospects for utilizing porteresia coarctata in rice breeding programs created date.
Department of agriculture agricultural research service. It is a wild relative of rice which can survive salinity up to 3040 dsm ref. Intergeneric somatic hybrids of rice oryza sativa l. The molecular basis of salt tolerance of lmyoinositol 1p synthase mips. Notable herbaceous elements such as suaeda maritima, suaeda nudiflora, salicornia brachiata, tylophora tenuisima. Functional screening of genes from a halophyte wild rice relative. Scientists develop salt tolerant rice variety the hindu. Salt tolerance in the halophytic wild rice, porteresia. Total rna was isolated from mature leaves of oryza and porteresia following the method of ostrem et al. Antifeeding and toxic effects of chemical extracts from porteresia coarctata takeoka on the insect pest spodoptera litura f. Pdf a gene coding for the chloroplastic fructose 1,6bisphosphatase pccfr in porteresia coarctata tateoka roxb. A group of indian scientists has developed a new salttolerant transgenic rice plant by overexpressing a gene from a wild rice called porteresia coarctata.
Samples were collected during the period of low tide from the bakkhali estuary at two stations. Salinity poses a serious threat to yield performance of cultivated rice in south asian countries. To change the order of your pdfs, drag and drop the files as you want. It is a form of wild rice that grows in saline estuaries in bangladesh. It is one of the species of haworthiopsis that is commonly cultivated as an ornamental description. Mangroves of mahanadi delta in the state of odisha and aspects of their conservation prasanna kumar nayak1, raj kishore mohanta2. The presence of pinitol synthesizing proteingene in a wild halophytic rice is remarkable, although its exact role in salt tolerance of p. We sequenced the transcriptome of porteresia under different conditions using illumina platform and generated about 375 million highquality reads. An international journal for rapid publication of reports on genes and genomes, volume 21.
Deep transcriptome sequencing of wild halophyte rice, porteresia. Then, based on his studies, tateoka 1963a,b, 1965 erected a new genus, porteresia, and transferred o. Characterization of porteresia coarctata beds along the goa. Porteresia coarctata, forms an important constituent in the plant succession leading to formation of mangrove communities along. Porteresia coarctata is a wild relative of rice with capability of high salinity and submergence tolerance. Micropropagation of porteresia coarctata tateoka, a wild relative of rice with useful genetic traits of salinity and submergence tolerance, was achieved t. Inositol methyl tranferase from a halophytic wild rice. Pcino1 and pcimt1, earlier isolated from porteresia coarctata were cloned into pcambia1 fig. The enhanced synthesis of pinitol in porteresia under stress may.
Micropropagation of porteresia coarctata tateoka, a wild relative of rice with useful genetic traits of salinity and submergence tolerance, was. Porteresia coarctata, the only halophyte wild rice, is a rich source of genes and promoters for improving salt tolerance in rice. Please, select more pdf files by clicking again on select pdf files. The transcriptome analyses of porteresia can lead to the identification of candidate genes involved in salinity and submergence tolerance. Isolation of a cdna clone pcsrp encoding serinerich. The filtered fastq files were further subjected to more stringent. Once you merge pdfs, you can send them directly to your email or download the file to our computer and view. In silico analysis confirms the absence of an imt1 homolog in oryza genome, and pcimt1 is.
Chloroplastic fructose1,6bis phosphatase, porteresia coarctata eu371994 iii. A gene coding for the chloroplastic fructose 1,6bisphosphatase pccfr in porteresia coarctata tateoka roxb. Porteresia coarctata oryza coarctata is a relative of oryza rice found in mangrove areas. Pdf characterization of progenies from intergeneric. Recalcitrant seed storage physiology in three aquatic. Porteresia coarctata syn oryza coarctata is a tetraploid wild rice growing abundantly in the coastal region of india and some other asian countries. Recalcitrant seed storage physiology in three aquatic grasses zizania palustris, spartina anglica and porteresia coarctata r. Phylogenetic analyses based on 16s rrna gene sequences indicated that the novel strains were.
Rearrange individual pages or entire files in the desired order. Spatiotemporal variation of stored carbon in porteresia coarctata along the east and west coasts of india 1. Two facultatively anaerobic, nitrogenfixing bacteria strains mssrf30t and mssrf31 were isolated from a mangroveassociated wild rice porteresia coarctata tateoka. In vitro propagation of salttolerant wild rice relative, porteresia. A cdna for porteresia coarctata inositol methyl transferase 1 pcimt1, coding for the inositol methyl transferase implicated in the synthesis of pinitol has been cloned from p. Agriculture and biodiversity international union for. Haworthiopsis coarctata, formerly haworthia coarctata, is a species of flowering succulent plant from the eastern cape province, south africa and naturalized in mexico. A novel salttolerant lmyoinositol1phosphate synthase. The distinct morphology and leaf architecture enabling the plant to exclude salt is a characteristic feature of porteresia in comparison. Tateoka desiccation intolerant, recalcitrant, despite the fact that o. Effect of salinity on nutritional value of saltmarsh grass porteresia coarctata from gangetic delta, northeast coast of india.
Mangroves are characterized by alkaline soils in which the halophytic wild rice relative porteresia coarctata thrives. Effect of salinity on nutritional value of saltmarsh grass. Seed development, the accumulation of abscisic acid and. Oryza coarctata in the germplasm resources information network grin, u. A concentration of 1,000 and 2,000 ppm extract exhibited significant. Pdf the possible overlap between salinity and mechanical. Longterm exposure to combined treatment of elevated co2. Plant promoter prediction tool and isolation of a promoter. If this is the first time you use this feature, you will be asked to authorise cambridge core to connect with your account. A phylogeny of the rice tribe oryzeae poaceae based on. Porteresia coarctata is a species of grass in the family poaceae, native to india, sri lanka. Porteresia coarctata is a species of grass in the family poaceae, native to india, sri lanka, bangladesh, and myanmar porteresia coarctata is considered by some botanists to be the only species in the genus porteresia.
Myriostachya wightiana and porteresia coarctata in muddy flats. Pdf cloning, characterization and expression of a chloroplastic. A 37amino acid stretch between trp174 and ser210 has been confirmed. It is noteworthy that porteresia coarctata is closely related to oryza species, suggesting that it should be treated as a member in the genus oryza rather than a separate monotypic genus. Tateoka is an endemic halophyte growing all over the coastal belt of bangladesh, propagating through rhizomes and setting a few rice. Porteresia coarctata phosphoenolpyruvate carboxylase eu371116 v. Other authors maintain instead that it should belong in the rice genus oryza, as oryza coarctata roxb. Deep transcriptome sequencing of wild halophyte rice. Once files have been uploaded to our system, change the order of your pdf documents. Porteresia coarctata is a mangrove rice, which being wild. Porteresia coarctata, the only halophyte wild rice, is a rich source of genes and. These strains were determined to be nitrogenfixers using the acetylene reduction assay and by pcr detection of a nifh gene amplicon. Using illumina and nanopore reads, we achieved the assembled genome size of 569.
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